a172 human glioblastoma cell lines  (Procell Inc)

Journal: CNS Oncology

Article Title: Arginine deprivation induces prolonged autophagy activation and ROS-dependent cell death in glioblastoma multiforme

doi: 10.1080/20450907.2026.2666026

Figure Lengend Snippet: Cytotoxicity of GBM cell lines A172 and U251 following prolonged arginine deprivation (A). Average ASS-1 levels, expressed as mean fluorescence intensity (MFI), for each condition (isotype, untreated and treated cells) at 24, 48, 72, 96, and 120 hours post-treatment. Stars indicate a significant increase in ASS-1 expression compared to both isotype control and untreated cells (B) ASS-1 expression of A172 and U251 cell lines at 72 hours post-treatment. MFI is on the X-axis (FL1-H) and forward scatter is on the Y-axis (C).

Article Snippet: Human Glioblastoma cell lines A172 and U251 MG were obtained from the American Type Culture Collection (A172 ATCC, CRL-1620) and from the European Collection of Authenticated Cell Cultures (U251 MG, ECACC, catalog # 09063001).

Techniques: Fluorescence, Expressing, Control

Journal: CNS Oncology

Article Title: Arginine deprivation induces prolonged autophagy activation and ROS-dependent cell death in glioblastoma multiforme

doi: 10.1080/20450907.2026.2666026

Figure Lengend Snippet: Autophagosome accumulation in GBM cells, expressed as mean fluorescence intensity (MFI), for control untreated cells and cells treated with [HuArgI (Co)-PEG5000] at 24, 48, 72, 96, and 120 hours post-treatment. Stars indicate a significant increase in autophagosome accumulation compared to untreated cells (A). Autophagosome accumulation in A172 and U251 cell lines at 72 hours post-treatment in untreated cells and cells treated with CQ, [HuArgI (Co)-PEG5000] and a combination of both. MFI is on the X-axis (FL1-H) and forward scatter is on the Y-axis (B). Immunohistochemistry staining of autophagosomes in A172 cells untreated and treated with CQ, [HuArgI (Co)-PEG5000], or a combination of both, at 24, 48, and 72 hours, post-treatment (C).

Article Snippet: Human Glioblastoma cell lines A172 and U251 MG were obtained from the American Type Culture Collection (A172 ATCC, CRL-1620) and from the European Collection of Authenticated Cell Cultures (U251 MG, ECACC, catalog # 09063001).

Techniques: Fluorescence, Control, Immunohistochemistry, Staining

Journal: CNS Oncology

Article Title: Arginine deprivation induces prolonged autophagy activation and ROS-dependent cell death in glioblastoma multiforme

doi: 10.1080/20450907.2026.2666026

Figure Lengend Snippet: Western blot of Atg13 and phosphor-Atg13 on protein extracts of A172 and U251 untreated and treated with [HuArgI (Co)-PEG5000] for up to 96 hours (Atg13) (A). Histogram of the ratio of p-Atg13 over Atg13 showing a significant decrease in the phosphorylation of Atg13 at 72- and 96-hours post-treatment. Stars indicate statistical significance (A). ULK phosphorylation levels expressed as mean fluorescence intensity (MFI), for untreated cells and cells treated with [HuArgI (Co)-PEG5000] at 24, 48, 72, 96, and 120 hours post-treatment. Double stars indicate significantly lower ULK phosphorylation in treated compared to untreated cells (B). ULK phosphorylation is also shown in A172 and U251 cell lines at 24- and 48-hours post-treatment in isotype controls, untreated cells and cells treated with [HuArgI (Co)-PEG5000]. MFI is represented on the X-axis (FL1-H) and forward scatter is represented on the Y-axis. Last panel on the right is a histogram representing the overlayed phospho-ULK MFI of the isotype control (red), untreated cells (black) and cells treated with [HuArgI (Co)-PEG5000] (blue) (B). Cytotoxicity of [HuArgI (Co)-PEG5000] alone and in combination with chloroquine (CQ) (50 µM) to A172 and U251 cells at 48, 72, 96, and 120 hours (C).

Article Snippet: Human Glioblastoma cell lines A172 and U251 MG were obtained from the American Type Culture Collection (A172 ATCC, CRL-1620) and from the European Collection of Authenticated Cell Cultures (U251 MG, ECACC, catalog # 09063001).

Techniques: Western Blot, Phospho-proteomics, Fluorescence, Control

Journal: CNS Oncology

Article Title: Arginine deprivation induces prolonged autophagy activation and ROS-dependent cell death in glioblastoma multiforme

doi: 10.1080/20450907.2026.2666026

Figure Lengend Snippet: Intracellular ROS accumulation in GBM cells following treatment with [HuArgI (Co)|-PEG5000], expressed as both percent positive cells (left panel) and mean fluorescence intensity (MFI) (right panel). Stars indicate a significant increase in ROS accumulation. The bottom histograms show ROS accumulation in treated cells (red) compared to controls (black) in A172 and U251 cells. (A) Autophagosome accumulation in GBM cells, expressed as MFI, for control untreated cells and cells treated with [HuArgI (Co)-PEG5000] alone or a combination of [HuArgI (Co)-PEG5000] and N-acetylcysteine (NAC) at 24, 48, 72, 96, and 120 hours post-treatment (B). Autophagosome formation in A172 and U251 cell lines. Control untreated cells (left panel) and cells treated with [HuArgI (Co)-PEG5000] (10 −7 M) (middle panel) or treated with [HuArgI (Co)-PEG5000] and NAC (0.82 g/L) (right panel) at 24, 48, 72, 96, and 120-hours post-treatment. In the histogram (right panel), control cells are in black, cells treated with [HuArgI (Co)-PEG5000] are in red and cells treated with the combination with NAC are in blue (C). Cytotoxicity of A172 and U251 cells treated with [HuArgI (Co)-PEG5000] alone or in combination with N-acetylcysteine (NAC) (0.82 g/L) (D).

Article Snippet: Human Glioblastoma cell lines A172 and U251 MG were obtained from the American Type Culture Collection (A172 ATCC, CRL-1620) and from the European Collection of Authenticated Cell Cultures (U251 MG, ECACC, catalog # 09063001).

Techniques: Fluorescence, Control

Journal: eLife

Article Title: Glial betaPix is essential for blood vessel development in the zebrafish brain

doi: 10.7554/eLife.106665

Figure Lengend Snippet: ( A ) qRT-PCR analysis showing that IPA3 treatment rescued stmn1a and stmn4l expression in CRISPR-mediated betaPix F 0 knockouts at 48 hpf. Each dot represents one embryo. Data are presented in mean ± SEM; one-way ANOVA with Dunnett’s test, individual p-values mentioned in the figure. ( B ) Schematic diagram illustrates four-guide RNAs-mediated F 0 knockout strategy in the loci of stmn1a , stmn1b, and stmn4l . ( C ) Whole-mount RNA in situ hybridization confirming that stmn1a, stmn1b, and stmn4l decreased in CRISPR-mediated stmn1a/1b/4 l F0 knockouts at 48 hpf. Dorsal views with anterior to the left. ( D ) qRT-PCR analysis confirming that stmn1a, stmn1b, and stmn4l decreased in mutant embryos of CRISPR-mediated stmn1a/1b/4 l F0 knockouts at 48 hpf. Each dot represents one embryo. Data are presented in mean ± SEM; unpaired Student’s t -test with individual p-values mentioned in the figure. ( E ) qRT-PCR analysis confirming that stmn1a, stmn1b, and stmn4l decreased in the FACS-sorted glia of CRISPR-mediated stmn1a/1b/4 l F0 knockouts at 48 hpf. Each dot represents sorted cells from one embryo. Data are presented in mean ± SEM; unpaired Student’s t -test with individual p-values mentioned in the figure. ( F ) Optical sections of glial structure in the hindbrains of siblings and CRISPR-mediated stmn1a/1b/4 l F0 knockouts at 48 hpf. Lateral view with anterior to the left. Abnormal glial structures with disoriented arrangements (yellow arrows) in stmn1a/1b/4l mutant embryos. ( G ) Quantification of average CtA and glial length in . Each dot represents one embryo. Data are presented in mean ± SEM; unpaired Student’s t -test with individual p-values mentioned in the figure. ( H ) Whole-mount RNA in situ hybridization revealed that nestin increased while pax2a decreased in CRISPR-mediated stmn1a/1b/4 l F0 knockout embryos at 48 hpf. Dorsal views with anterior to the left. ( I ) 3D reconstruction and whole-mount RNA in situ hybridization showing that gfap:GFP-stmn1b transgenic overexpression rescued glial defects in bbh fn40a ; Tg( gfap:GFP-stmn1b ) embryos at 48 hpf. Lateral views with anterior to the left. ( J ) Whole-mount RNA in situ hybridization showing that nestin and pax2a were normally expressed in bbh fn40a ; Tg( gfap:GFP-stmn1b ) embryos at 48 hpf. Dorsal views with anterior to the left. ( K ) Quantification of average CtA and glia length in . Each dot represents one embryo. Data are presented in mean ± SEM; unpaired Student’s t -test with individual p-values mentioned in the figure. ( L ) qRT-PCR analysis confirming the efficacy of betaPix transgenic overexpression and betaPix siRNA knockdown in U251 cells. Data are presented in mean ± SEM; one-way ANOVA with Dunnett’s test, individual p-values mentioned in the figure. ( M ) Representative stereomicroscopy images of A172 cells at 0 and 18 hours after wounding. betaPIX siRNA treatment decreased A172 cell migration, which was rescued by either betaPIX or STMN1 overexpression. The wound edges are highlighted by dashed lines, with arrow lines indicating the wound width. ( N ) Quantification of wound closures in ( M ). Data are presented in mean ± SEM; one-way ANOVA with Dunnett’s test. ** p<0.01 compared to negative control siRNA and empty vector transfection. ## p<0.01, ### P <0.005 compared to betaPix knockdown and empty vector transfection. Individual scale bars are indicated in the figure.

Article Snippet: U251 and A172 human glioblastoma cell lines, kindly provided by Dr. Jian Chen at Chinese Institute for Brain Research, Beijing, were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China), cultured in high glucose DMEM medium (Hyclone) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin, at 37°C in 5% CO 2 humidified incubator.

Techniques: Quantitative RT-PCR, Expressing, CRISPR, Knock-Out, RNA In Situ Hybridization, Mutagenesis, Transgenic Assay, Over Expression, Knockdown, Migration, Negative Control, Plasmid Preparation, Transfection

Journal: ACS Applied Materials & Interfaces

Article Title: Hyaluronic Acid-Coated SPIONs with Attached Folic Acid as Potential T2 MRI Contrasts for Anticancer Therapies

doi: 10.1021/acsami.4c20101

Figure Lengend Snippet: MTT test results for (A) A172 cells treated with SPION/HA, (B) A172 cells treated with SPION/HA-FA, (C) SW620 cells treated with SPION/HA, and (D) SW620 cells treated with SPION/HA-FA. Results are presented as mean ± SD ( n = 3), as a percentage relative to the control.

Article Snippet: The human glioblastoma multiforme A172 cell line (ATCC) and human adenocarcinoma SW620 cell line (ATCC) were cultured in a high-glucose Dulbecco’s modified Eagle’s (DMEM) medium, supplemented with 10% (v/v) FBS and 1% penicillin–streptomycin at 37 °C and 5% CO 2 .

Techniques: Control

Journal: ACS Applied Materials & Interfaces

Article Title: Hyaluronic Acid-Coated SPIONs with Attached Folic Acid as Potential T2 MRI Contrasts for Anticancer Therapies

doi: 10.1021/acsami.4c20101

Figure Lengend Snippet: Confocal laser scanning microscope images of A172 cells, incubated with FITC-labeled SPION/HA or SPION/HA-FA. Control: fluorescence image (40× objective) of the control cells with stained cell nuclei (blue fluorescence) and F-actin (red fluorescence); SPION/HA: fluorescence image (40× objective) of the cells with stained F-actin (red fluorescence) after incubation with FITC-labeled SPION/HA (green fluorescence); SPION/HA-FA: fluorescence image (100× objective) of the cells with stained F-actin (red fluorescence) after incubation with FITC-labeled SPION/HA-FA (blue and green fluorescence).

Article Snippet: The human glioblastoma multiforme A172 cell line (ATCC) and human adenocarcinoma SW620 cell line (ATCC) were cultured in a high-glucose Dulbecco’s modified Eagle’s (DMEM) medium, supplemented with 10% (v/v) FBS and 1% penicillin–streptomycin at 37 °C and 5% CO 2 .

Techniques: Laser-Scanning Microscopy, Incubation, Labeling, Control, Fluorescence, Staining

Journal: Microbes and infection

Article Title: HERV-W envelope protein is present in microglial cells of the human glioma tumor microenvironment and differentially modulates neoplastic cell behavior.

doi: 10.1016/j.micinf.2024.105460

Figure Lengend Snippet: Fig. 1. HERV-W ENV is present in human glioma tissue. (A–D) Anti-HERV-W ENV DAB immunostainings of non-neoplastic control brain tissue (A), oligoden droglioma, IDH-mutant and 1p/19q-codeleted, CNS WHO grade 2 (B), glioblastoma, IDH-wildtype, CNS WHO grade 4 (C) and oligodendroglioma, IDH-mutant and 1p/19q-codeleted, CNS WHO grade 3 (D). See insets for more tissue detail. Scale bars: 100 μm. (E) Real-time RT PCR of 32 diffuse gliomas (cumulated and according to CNS WHO grades 2 (II), 3 (III), and 4 (IV)) and normal brain (NB). (F–H) Immunofluorescent double staining of an IDH-wildtype glioblastoma (HERV-W ENV: green; CD3/GFAP/IBA1: red). Scale bars: 100 μm (A–D), 50 μM, and 20 μM for related insets (F–H). Data are shown as means (±SEM). Statistical significance was calculated using Welch’s ANOVA with Dunnett’s T3 multiple comparisons test. Data were considered significantly different at **p ≤0.01, ***p ≤0.001.

Article Snippet: Human glioblastoma (GBM) cell lines A172, LN229, and T98G (obtained from American Type Culture Collection; ATCC) were cultured in T-75 flasks with GBM culture medium consisting of Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher Scientific, Waltham, UK) supplemented with 10 % fetal bovine serum (FBS; Capricorn Scientific, Palo Alto, CA, USA) and 50 U/ml penicillin/streptomycin (Invitrogen, Carlsbad, USA) maintained in a 5 % CO2 atmosphere at 37 ◦C, following ATCC’s guidelines.

Techniques: Control, Mutagenesis, Quantitative RT-PCR, Double Staining

Journal: Microbes and infection

Article Title: HERV-W envelope protein is present in microglial cells of the human glioma tumor microenvironment and differentially modulates neoplastic cell behavior.

doi: 10.1016/j.micinf.2024.105460

Figure Lengend Snippet: Fig. 2. HERV-W ENV stimulation results in a microglia-dependent modulation of human glioblastoma cell line gene expression. (A–C) Human glioblastoma cell lines T98 (A), A172 (B), and LN229 (C) were stimulated with either HERV-W ENV or buffer in the absence (-MG) or presence (+MG and *MG) of rat microglia for 1, 3 or 5 days, respectively. Data are presented as z-scores and were considered significantly different at *p ≤0.05, **p ≤0.01, and ***p ≤0.001 assessed with the one sample t-test.

Article Snippet: Human glioblastoma (GBM) cell lines A172, LN229, and T98G (obtained from American Type Culture Collection; ATCC) were cultured in T-75 flasks with GBM culture medium consisting of Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher Scientific, Waltham, UK) supplemented with 10 % fetal bovine serum (FBS; Capricorn Scientific, Palo Alto, CA, USA) and 50 U/ml penicillin/streptomycin (Invitrogen, Carlsbad, USA) maintained in a 5 % CO2 atmosphere at 37 ◦C, following ATCC’s guidelines.

Techniques: Gene Expression

Journal: Microbes and infection

Article Title: HERV-W envelope protein is present in microglial cells of the human glioma tumor microenvironment and differentially modulates neoplastic cell behavior.

doi: 10.1016/j.micinf.2024.105460

Figure Lengend Snippet: Fig. 3. HERV-W ENV stimulation modulates the protein expression of human glioblastoma cell lines. (A-C′) Human glioblastoma cell lines T98 (A, A′), A172 (B, B′), and LN229 (C,C′) were stimulated with either HERV-W ENV or buffer in the presence (+MG) of rat microglia for 3 days. Data are shown as mean (±SEM) pixel densities of two biological replicates.

Article Snippet: Human glioblastoma (GBM) cell lines A172, LN229, and T98G (obtained from American Type Culture Collection; ATCC) were cultured in T-75 flasks with GBM culture medium consisting of Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher Scientific, Waltham, UK) supplemented with 10 % fetal bovine serum (FBS; Capricorn Scientific, Palo Alto, CA, USA) and 50 U/ml penicillin/streptomycin (Invitrogen, Carlsbad, USA) maintained in a 5 % CO2 atmosphere at 37 ◦C, following ATCC’s guidelines.

Techniques: Expressing

Journal: Microbes and infection

Article Title: HERV-W envelope protein is present in microglial cells of the human glioma tumor microenvironment and differentially modulates neoplastic cell behavior.

doi: 10.1016/j.micinf.2024.105460

Figure Lengend Snippet: Fig. 4. HERV-W ENV stimulation leads to microglia-dependent clustering of human glioblastoma cell lines. (A–C) Human glioblastoma cell lines T98 (A), A172 (B), and LN229 (C) were stimulated with either HERV-W ENV or buffer in the absence (-MG) or presence (+MG) of rat microglia for 3 or 5 days, respectively (F- actin: red; Iba1: green). All scale bars: 100 μm.

Article Snippet: Human glioblastoma (GBM) cell lines A172, LN229, and T98G (obtained from American Type Culture Collection; ATCC) were cultured in T-75 flasks with GBM culture medium consisting of Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher Scientific, Waltham, UK) supplemented with 10 % fetal bovine serum (FBS; Capricorn Scientific, Palo Alto, CA, USA) and 50 U/ml penicillin/streptomycin (Invitrogen, Carlsbad, USA) maintained in a 5 % CO2 atmosphere at 37 ◦C, following ATCC’s guidelines.

Techniques:

Journal: Microbes and infection

Article Title: HERV-W envelope protein is present in microglial cells of the human glioma tumor microenvironment and differentially modulates neoplastic cell behavior.

doi: 10.1016/j.micinf.2024.105460

Figure Lengend Snippet: Fig. 5. HERV-W ENV stimulation modulates the proliferation of glioblastoma cells but has no effect on apoptosis. (A–F) Human glioblastoma cell lines T98 (A–B), A172 (C–D), and LN229 (E–F) were stimulated with either HERV-W ENV or buffer in the absence (-MG; A,B,C) or presence (direct contact: +MG; A′,B′,C′ and transwell-separated: *MG; A″,B″,C″) of rat microglia for 1 or 5 days, respectively (Ki67: green; CC3: red). Statistical significance was calculated using the one sample t- test: *p ≤0.05, **p ≤0.01. Scale bars: 100 μm and 50 μm for insets (A–F).

Article Snippet: Human glioblastoma (GBM) cell lines A172, LN229, and T98G (obtained from American Type Culture Collection; ATCC) were cultured in T-75 flasks with GBM culture medium consisting of Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher Scientific, Waltham, UK) supplemented with 10 % fetal bovine serum (FBS; Capricorn Scientific, Palo Alto, CA, USA) and 50 U/ml penicillin/streptomycin (Invitrogen, Carlsbad, USA) maintained in a 5 % CO2 atmosphere at 37 ◦C, following ATCC’s guidelines.

Techniques:

Journal: Microbes and infection

Article Title: HERV-W envelope protein is present in microglial cells of the human glioma tumor microenvironment and differentially modulates neoplastic cell behavior.

doi: 10.1016/j.micinf.2024.105460

Figure Lengend Snippet: Fig. 6. HERV-W ENV stimulation results in a microglia-dependent increase of migration velocity of human glioblastoma cells. (A-C″) Human glioblastoma cell lines T98 (A-A‴), A172 (B–B″), and LN229 (C–C″) were stimulated with either 1000 ng/ml of recombinant HERV-W ENV or buffer in the absence (-MG) or presence (+MG) of rat microglia, respectively. Culture inserts were used to create a predefined gap of 500 μm and after 4, 8, 12, and 16h gap closure was measured. Data are shown as means (±SEM). Statistical significance was calculated using the Student’s two-sided, unpaired t-test: *p ≤0.05. Scale bars: 100 μm.

Article Snippet: Human glioblastoma (GBM) cell lines A172, LN229, and T98G (obtained from American Type Culture Collection; ATCC) were cultured in T-75 flasks with GBM culture medium consisting of Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher Scientific, Waltham, UK) supplemented with 10 % fetal bovine serum (FBS; Capricorn Scientific, Palo Alto, CA, USA) and 50 U/ml penicillin/streptomycin (Invitrogen, Carlsbad, USA) maintained in a 5 % CO2 atmosphere at 37 ◦C, following ATCC’s guidelines.

Techniques: Migration, Recombinant